Fig. 3

Vgll4b and Vgll4l are required to limit the number of cells in the pLLP.

Overview (a, c) and higher magnification images (b, b’, d, d’) taken on a brightfield microscope of 32 hpf cldnb:lynGFP embryos stained by in situ hybridization (ISH) for vgll4b (a, b) and vgll4l (c, d) and counterstained with an anti-GFP antibody (b’, d’). Schematic representation of WT Vgll4b and the mutant form Vgll4b^bns23 (e), and WT Vgll4l and the mutant form Vgll4l^fu93 (f). MIP of confocal Z-stacks showing the pLLP in vgll4b^+/+;vgll4l^+/+ (g), vgll4b^−/−;vgll4l^+/+ (h), vgll4b^+/+;vgll4l^−/− (i), vgll4b^−/−;vgll4l^+/− (j), vgll4b^+/−;vgll4l^−/− (k), vgll4b^−/−;vgll4l^−/− (l) embryos. m Quantifications of pLLP cell number for each indicated group (N ≥ 4 independent replicates). Data are presented as mean ± SD. Unpaired t-tests (Mann–Whitney) were conducted. Scale bar: 400 μm (a, c), 20 μm (b–d’, g–l).