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yap1 and vgll4b/l are required for proper formation of the pLLP. Single plane confocal images showing the amount of EdU-stained nuclei in comparison to cldnb:lynGFP signal in yap1+/+ (a–a”’), yap1−/− (b–b”’), vgll4b+/+;vgll4l+/+ (c–c”’), and vgll4b−/−;vgll4l−/− (d–d”’) embryos at 19 hpf. Quantification of pLLP cell number (e), EdU positive cell number (f), and ratio of EdU positive cell number to total pLLP cell number (g) in each condition (N = 1 independent replicate for yap1, N = 3 independent replicates for vgll4). Data are presented as mean ± SD. Unpaired t-tests (Mann–Whitney) were conducted. Scale bar: 20 μm (a–d”’).
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