FIGURE 2

Eye, head, and body measurements from control and crim1^−/− larvae that are homozygous for a 2 base pair deletion, c.339_340delCT p.Leu112Leufs*3, at 48 and 72 h post fertilization (hpf). (A) Control larva showing measurements obtained for eye diameter and head length. (B, C) Control larva showing site of measurements for eye diameter and body and tail length. (D) Measurements of eye diameter in control larvae (white columns) and crim1^−/− larvae (black columns) at 48 hpf and 72 hpf. Each dot represents a single measurement. Eye diameter is significantly decreased in the crim1^−/− larvae compared to controls. (E) Ratio of eye diameter to body length in control larvae (white columns) and crim1^−/− larvae (black columns) at 48 hpf and 72 hpf. Each dot represents a single measurement. The crim1^−/− larvae demonstrate a significantly increased ratio for eye diameter to body length compared to controls. (F) Ratio of eye diameter to head diameter in control and crim1^−/− larvae. Each gray dot represents a measurement at 24 hpf, 48 hpf, and 72 hpf. The height of the white columns represents the mean of the ratio of eye diameter to head diameter for control larvae and the height of the black columns representing the mean of the ratio of eye diameter to head diameter for the crim1^−/− larvae. The crim1^−/− larvae show a significantly increased ratio of eye diameter to head diameter at 48 hpf (P = 0.008), but the ratio was not significantly increased at 24 hpf or 72 hpf.