FIGURE

FIGURE 8

ID

ZDB-FIG-250411-8

Publication

Strachan et al., 2025 - Novel in vivo models of autosomal optic atrophy reveal conserved pathological changes in neuronal mitochondrial structure and function

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FIGURE 8

Analysis of mitochondrial respiration in an in vivo model of optic atrophy. (A) Schematic outlining the Mito Stress Test conducted in this study. Oxygen consumption rate (OCR) is measured prior to and following the additions of the ATP synthase inhibitor oligomycin, the uncoupling agent FCCP, and finally the inhibitors of complex I and III, rotenone and antimycin A (respectively). (B) OCR profiles of non‐injected control, buffer injected control, and Opa1 crispant <131 hpf larvae. (C–G) Graphs represent the mean ± SD OCR for the calculated basal respiration (C), ATP production (D), maximal respiration (E), non‐mitochondrial respiration (F) and proton leak (G). Statistical analysis throughout consists of one‐way ANOVA and Tukey's multiple comparisons tests.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	opa1^ucd16/ucd16
Observed In:	aerobic respiration
Stage:	Day 4

Phenotype Detail

Acknowledgments

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