FIGURE 5

Social behavior analysis of wild‐type and adap1 mutants in the adult stage. (a) Schematic representation of the social behavior test used in this study. Areas 3 and 5 were set as the region near the stimulus exploration. One and three novel conspecifics (e.g., albino line) were placed in chamber I and III, respectively. (b and c) The distance moved by zebrafish in chamber II and the time spent in each area (areas 3, 4, and 5) for each minute. The adap1^uy70/WT and adap1^uy70/uy70 swam longer than the wild type (WT). The adap1^uy70/uy70 stayed longer than the wild type in area 3. The adap1^uy71/WT stayed shorter than the wild type in area 3. The adap1^uy70/WT, adap1^uy70/uy70, and adap1^uy71/WT stayed shorter than the wild type in area 4. The adap1^uy70/WT, adap1^uy70/uy70, and adap1^uy71/WT stayed longer than the wild type in area 5. The numbers of zebrafish are Ν = 16 for WT/WT, Ν = 19 for WT/uy70, Ν = 17 for uy70/uy70, Ν = 30 for WT/WT, Ν = 34 for WT/uy71, Ν = 29 for uy71/uy71. *p < 0.05, **p < 0.01. (d and e) Heat map of the behavior of wild‐type and adap1 mutants. The numbers of zebrafish in each group are same in (b and c). A high number of color indicators indicates that zebrafish were frequently located at that position during the 20 min test period.