Fig. 5.

Staining of the lateral line and Purkinje neurons in zebrafish ebf3a mutants. a) Counts for lateral line neuromasts on one side of the 6 dpf zebrafish. Run 1 N = 15 −/− and 50 +/− and +/+; Run 2 N = 16 −/− and 36 +/− and +/+. Significance was assessed using an unpaired t-test. b) Representative image of lateral line staining. The white arrow indicates the MI2 neuromast which was absent in some homozygous mutant larvae. c) Combined quantification of missing MI2 neuromasts from both runs. Only homozygous mutants and WT siblings were included. One-sided Chi-square test: P = 0.0218. d) Brain map of the quantified differences in Parvalbumin staining, comparing homozygous mutants (−/−) to WT (+/+) siblings. A sum-of-slices intensity projection is shown (Z- and X- axes) of the signals inside the brain (white outline). N = 14 homozygotes (−/−) compared with 26 wild types (+/+). e) Example slice of Parvalbumin staining from registered stacks. The slice is at position 100 out of 138 in the standard Z-Brain stack. The cerebellum region is boxed and shown enlarged to the right.