FIGURE

Figure 9

ID

ZDB-FIG-260418-22

Publication

Patel et al., 2026 - Cdhr1a and pcdh15b may link photoreceptor outer segments with calyceal processes revealing a potential mechanism for cone-rod dystrophy

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Figure 9

Cone phenotypes are exacerbated by simultaneous loss of pcdh15b and cdhr1a.
(A–D) Confocal microscopy of retinal cryosections from 5 dpf pcdh15b^-/-, cdhr1a^-/-, cdhr1a^-/-; pcdh15b^+/- and cdhr1a^-/-; pcdh15b^-/- larva probed with prph2 antibodies (green). COS = cone outer segment. B=blue cones, RG = red/green cones, UV = UV cones. Scale bar = 10 μm. (E–H) Confocal microscopy of retinal cryosections from 10 dpf pcdh15b^-/-, cdhr1a^-/-, cdhr1a^-/-; pcdh15b^+/- and cdhr1a^-/-; pcdh15b ^-/- larva probed with prph2 antibodies (green). COS = cone outer segment. B=blue cones, RG = red/green cones, UV = UV cones. Scale bar = 10 μm. (I) Quantification of cone outer segment length at 5 dpf based on prph2 signal. ns = not significant, ****=p < 0.0001. ANOVA = p < 0.0001. (J) Quantification of cone outer segment length at 10 dpf based on prph2 signal. ns = not significant, **=p < 0.01, ****=p < 0.0001. ANOVA = p < 0.0001. (K–N) Confocal microscopy of retinal cryosections from 5 dpf pcdh15b^-/-, cdhr1a^-/-, cdhr1a^-/-; pcdh15b^+/- and cdhr1a^-/-; pcdh15b ^-/- larva probed with actin antibodies (white). COS = cone outer segment. Scale bar = 10 μm. (O–R) Confocal microscopy of retinal cryosections from 10 dpf pcdh15b^-/-, cdhr1a^-/-, cdhr1a^-/-; pcdh15b^+/- and cdhr1a^-/-; pcdh15b^-/-larva probed with actin antibodies (white). COS = cone outer segment. Scale bar = 10 μm. (R) Quantification of cone CP length at 5 dpf based on prph2 signal. ns = not significant, ****=p < 0.0001. ANOVA = p < 0.0001. (T) Quantification of cone CP length at 10 dpf based on prph2 signal. ns = not significant, *=p < 0.05 **=p < 0.01****,=p < 0.0001. ANOVA = p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdhr1a^{uky203/uky203} pcdh15b^{uky204/uky204} pcdh15b^uky204/+ cdhr1a^{uky203/uky203}; pcdh15b^uky204/+ cdhr1a^{uky203/uky203}; pcdh15b^{uky204/uky204}
Observed In:	retinal cone cell cone photoreceptor outer segment
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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