FIGURE

Figure 6

ID

ZDB-FIG-260623-15

Publication

Capecki et al., 2026 - Evidence that disruption of Discoidin domain receptor 2 contributes to palate malformations through effects on the extracellular matrix

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Figure 6

Differential expression of leading-edge extracellular matrix (ECM) genes in 4 dpf ddr2a CRISPR-F0 zebrafish larvae (zfl) heads compared with scrambled controls (scr-ctrl). (A) volcano plot showing log₂ fold change versus –log₁₀ adjusted p-value for ECM-related leading-edge genes identified by absolute gene-set enrichment analysis (GSEA). Each dot represents one gene. The dashed vertical line marks no change in expression, and the horizontal dashed line indicates the significance cutoff (p_adj < 0.05). In total, 62 of 71 significantly dysregulated genes were down-regulated in ddr2a F0-KOs, 9 were up-regulated. (B) Heatmap of log₂ fold changes for the top 30 leading-edge genes identified by absolute GSEA in the comparison of ddr2a F0-KO versus scrambled control (P = 0.049). Gene expression changes were derived from DESeq2 differential expression analysis. Colors indicate the direction and magnitude of the expression.

Expression Data

Genes:	adamts13 agt angptl3 anxa2b c1qtnf9 cela1.6 ctsbb ctslb ebi3 ecm1a f7i fga fgb fgg he1.1 he1.2 ins itih3b.1 itln2 lgals2b mbl2 mep1a.1 mep1a.2 prg4b prss1 s100a10a serpina1 serpinf2a serpinh1b tmprss15
Fish:	WT + CRISPR1-ddr2a + CRISPR2-ddr2a + CRISPR3-ddr2a + CRISPR4-ddr2a + CRISPR5-ddr2a
Knockdown Reagents:	CRISPR1-ddr2a CRISPR2-ddr2a CRISPR3-ddr2a CRISPR4-ddr2a CRISPR5-ddr2a
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-ddr2a + CRISPR2-ddr2a + CRISPR3-ddr2a + CRISPR4-ddr2a + CRISPR5-ddr2a
Knockdown Reagents:	CRISPR1-ddr2a CRISPR2-ddr2a CRISPR3-ddr2a CRISPR4-ddr2a CRISPR5-ddr2a
Observed In:	extracellular matrix assembly whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.