Fig. 6

Effect of blocking peptide BP2 on Pkd2-dependent disease phenotypes in larval zebrafish.
A Similar experimental conditions as in Fig. 3C. Distribution of the four tail curling severities under four different experimental conditions: with or without Pkd2 KD, RASSF4 OE, and BP2 OE, as indicated, with “Ctrl” indicating water injection. The total number of zebrafish used under each condition, from three independent experiments, is indicated. BP2 OE: micro-injection of 200 pg BP2 mRNA. ****p < 0.0001, by chi-square test. B Survival rates of different groups of larval zebrafish over an 8-day period. C Similarly as in Fig. 3F, the averaged cyst-like occurrence % obtained in 5-dpf zebrafish under the indicated conditions from three independent experiments, with 15–30 fish used for calculating the cyst-like occurrence % for each condition in each experiment. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, by unpaired Student’s t test.